2. Signaling Pathways
  3. Cell Cycle/DNA Damage
  4. DNA/RNA Synthesis

DNA/RNA Synthesis

DNA/RNA Synthesis

Related Products

PDF 1.61 MB

RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.

Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.

First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.

DNA/RNA Synthesis Isoform Specific Products:

DNA/RNA Synthesis Isoform Comparison
Cat. No. Product Name Effect Purity Chemical Structure
  • HY-143274
    DENV-IN-5
    		Inhibitor
    DENV-IN-5 (Compound 4b) is a dengue virus (DENV) inhibitor with EC50s of 1.47, 9.23, 7.08 and 8.91 μM against DENV-I ∼ IV replication, respectively. DENV-IN-5 also inhibits HIV-1IIIB strain with an EC50 of 0.1512 μM.
    DENV-IN-5
  • HY-150247
    RNA splicing modulator 2
    		Modulator
    RNA splicing modulator 2 (compound 256) is a RNA splicing modulator.
    RNA splicing modulator 2
  • HY-132138
    5-Propargylamino-3'-azidomethyl-dCTP
    		Chemical
    5-Propargylamino-3'-azidomethyl-dCTP is a nucleoside molecule extracted from patent WO2004018497A2, compound 17. 5-Propargylamino-3'-azidomethyl-dCTP can be used in DNA synthesis and DNA sequencing. 5-Propargylamino-3'-azidomethyl-dCTP is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    5-Propargylamino-3'-azidomethyl-dCTP
  • HY-181459
    PARP1-IN-55
    		Inhibitor
    PARP1-IN-55 is a potent and selective PARP1 inhibitor with an IC50 value of 0.019 μM. PARP1-IN-55 exhibits anti-proliferative selective activity against MCF-7 breast cancer cells (IC50 = 3.6 μM). PARP1-IN-55 inhibits the PARP1-mediated DNA damage repair pathway, induces ROS accumulation, disrupts mitochondrial membrane potential, induced apoptosis and suppresses cancer cell migration, invasion, and colony formation. PARP1-IN-55 can be used for the study of breast cancer.
    PARP1-IN-55
  • HY-W013068S1
    DMT-dT Phosphoramidite-15N2
    DMT-dT Phosphoramidite-15N2 is the 15N labeled DMT-dT Phosphoramidite. DMT-dT Phosphoramidite is typically used in the synthesis of DNA.
    DMT-dT Phosphoramidite-<sup>15</sup>N<sub>2</sub>
  • HY-178022
    HDAC6-IN-63
    		Inhibitor
    HDAC6-IN-63 (Compound 7) is an orally active HDAC6 inhibitor with an IC50 of 145  nM. HDAC6-IN-63 inhibits the expression of Sp1 and RAD51, thereby inducing Caspase-dependent apoptosis. HDAC6-IN-63 has antitumor activity and sensitizes Etoposide (HY-13629) and Gemcitabine (HY-17026), promoting synergistic death of NSCLC cells through the inhibition of homologous recombination and non-homologous end joining (NHEJ) pathways involved in DNA DSB repair. HDAC6-IN-63 can be used for chemotherapy of cancers like NSCLC research.
    HDAC6-IN-63
  • HY-182322
    POLQ-IN-1
    		Inhibitor
    POLQ-IN-1 is a DNA polymerase θ (POLQ) inhibitor with a pIC50 of 8.2. POLQ-IN-1 inhibits the proliferation of homologous recombination-deficient cells. POLQ-IN-1 is used for the research of homologous recombination-deficient tumors.
    POLQ-IN-1
  • HY-P11334
    Cyanostatin B
    		Inhibitor
    Cyanostatin B, a cyanobacterial lipopeptide, is a leucine aminopeptidase M (LAP) inhibitor (IC50 = 12 ng/mL). Cyanostatin B is a weak inhibitor of protein phosphatase (PP2A) and also exhibits weak inhibitory activity against angiotensin-converting enzyme (ACE), with an IC50 value of 130 μg/mL. Cyanostatin B demonstrates both cytotoxic and genotoxic effects on human hepatocytes, although non-toxic to Artemia salina. Cyanostatin B inhibits the proliferation of HepG2 cells, induces DNA single-strand breaks, and causes genomic instability..
    Cyanostatin B
  • HY-E70585
    T4 Phage β-glucosyltransferase
    T4 Phage β-glucosyltransferase is a DNA-modifying enzyme encoded by bacteriophage T4. T4 Phage β-glucosyltransferase transfers glucose from uridine diphosphoglucose to 5-hydroxymethyl cytosine bases of phage T4 DNA.
    T4 Phage β-glucosyltransferase
  • HY-114461B
    (3S,17S)-FD-895
    (3S,17S)-FD-895 a FD-895 (HY-114461) analogue, is a spliceosome modulator. (3S,17S)-FD-895 inhibits cancer cell growth and exhibits gene-selective splice modulatory activity. (3S,17S)-FD-895 can be used for the research of colorectal carcinoma.
    (3S,17S)-FD-895
  • HY-N0667S7
    L-Asparagine-13C4,15N2
    L-Asparagine-13C4,15N2 ((-)-Asparagine-13C4,15N2) is the 13C and 15N-labeled L-Asparagine (HY-N0667). L-Asparagine is an essential amino acid for leukemic cells and a substrate for L-Asparaginase. L-Asparaginase is a potent anti-leukemic enzyme that promotes asparagine (Asn) and glutamine (Gln) depletion and inhibits protein biosynthesis in lymphoblasts. Removal of L-asparagine from plasma by L-Asparaginase results in inhibition of RNA and DNA synthesis and subsequent apoptosis. L-Asparaginase has cell-killing ability in vitro and in vivo, and selectively inhibits the growth of cancer cells with low asparagine synthetase (AASNS) expression. L-Asparagine can be used as a biomarker and sensor for the study of childhood acute lymphoblastic leukemia.
    L-Asparagine-<sup>13</sup>C<sub>4</sub>,<sup>15</sup>N<sub>2</sub>
  • HY-P11680
    Boc-PNA-thioU(PMB)-OH
    BOC-PNA-thioU(PMB)-OH is a peptide nucleic acid (PNA) monomer characterized by a PMB protecting group on its thiouracil, which enhances its binding affinity to adenine. BOC-PNA-thioU(PMB)-OH exhibits excellent resistance to enzymatic degradation and strong hybridization properties, making it valuable for applications in gene diagnosis, antisense therapy, and other molecular biology fields requiring higher binding strength and greater functionalization.
    Boc-PNA-thioU(PMB)-OH
  • HY-134724
    MTH1 ligand 1
    MTH1 ligand 1 is a target protein ligand for MTH1 and can be used to synthesize PROTAC aTAG 2139 (HY-161162).
    MTH1 ligand 1
  • HY-168933
    KWR095
    		Inhibitor
    KWR095 is an orally active WRN inhibitor with an IC50 of 0.032 μM for WRN ATPase. KWR095 can impede the duplex unwinding activity of WRN and inhibit the proliferation of tumor cells. KWR095 has anti-tumor activity.
    KWR095
  • HY-13550A
    Ametantrone acetate
    		Inhibitor
    Ametantrone acetate (NSC 196473 acetate) is an antineoplastic drug with topoisomerase II inhibitory activity. Ametantrone acetate causes covalent cross-linking of DNA. The pharmacokinetic profile of Ametantrone acetate has been confirmed in clinical trials, and its elimination pathway in the body indicates the existence of a major metabolic pathway.
    Ametantrone acetate
  • HY-16035A
    Alatrofloxacin mesylate
    		Inhibitor
    Alatrofloxacin mesylate is an antibiotic with antibacterial activity. Alatrofloxacin mesylate exhibits significant hydrophilicity in the aqueous phase, forming a very stable suspension. The degradation products of Alatrofloxacin mesylate become hydrophobic upon exposure, causing aggregation of its particles. The long-term stability of Alatrofloxacin mesylate is closely related to its polar interaction. The changes in surface tension characteristics of Alatrofloxacin mesylate particles under different lighting conditions affect their physical and chemical properties.
    Alatrofloxacin mesylate
  • HY-14716
    CCT239065
    CCT239065 is an orally available, effective inhibitor of the mutant protein kinase V600EBRAF (RAF) with anti-tumor activity. CCT239065 inhibits downstream signaling of V600EBRAF in cancer cells, blocking DNA synthesis and suppressing proliferation.
    CCT239065
  • HY-14509
    LP-661438
    		Inhibitor
    LP-661438 (Compound 10) is an orally active DCK inhibitor (IC50 = 1.7 nM). LP-661438 inhibits deoxycytidine kinase in T cells and in vivo.
    LP-661438
  • HY-N3497
    Isochamaejasmin
    		Inhibitor
    Isochamaejasmin is a biflavonoid with anti-cancer, antiplasmodial and insecticidal activities. Isochamaejasmin displays a potent NF-κB (NF-κB) activation activity. Isochamaejasmin could cause DNA damage and induce apoptosis via the mitochondrial pathway in AW1 cells. Isochamaejasmin also has a moderate antiplasmodial activity (IC50 of 7.3 μM for P. falciparum) and relatively low cytotoxicity (CC50 of 29.0 μM).
    Isochamaejasmin
  • HY-181850
    Telomeric G4s ligand 2
    		Inhibitor
    Telomeric G4s ligand 2 is an orally active, selective ligand of telomeric G-quadruplex (G4), with an IC50 of 0.4 μM. Telomeric G4s ligand 2 binds to dimeric telomeric G4, inhibits the activities of DHX36 and BLM helicases. Telomeric G4s ligand 2 activates cGAS-STING and TERRA-ZBP1 pathways, inducing autophagy and G2/M cell cycle arrest, and exhibits antiproliferative effects across cancer cell lines. Telomeric G4s ligand 2 can be used for the study of colorectal cancer.
    Telomeric G4s ligand 2
Cat. No. Product Name / Synonyms Application Reactivity
DNA/RNA Synthesis Isoform Comparison

Your Search Returned No Results.

Sorry. There is currently no product that acts on isoform together.

Please try each isoform separately.